Volume 6, Issue 11 (11-2018)                   ifej 2018, 6(11): 14-20 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Khoshhal Sarmast M, Mosavizadeh S J, Sharifani M. (2018). Evaluation of Junipers spp. Genetic Diversity in Northern Iran using ISSR Markers . ifej. 6(11), 14-20. doi:10.29252/ifej.6.11.14
URL: http://ifej.sanru.ac.ir/article-1-241-en.html
Abstract:   (3440 Views)
The aim of the present work was to study genetic diversity within and among of five native populations of Juniperus spp. in three provenances of Golestan, Mazandran and Gilan. Four out of 15 ISSR primers have yielded 285 scorable, polymorphic fragments in 32 accessions, which were utilized to estimate genetic diversity. Analysis of molecular variance with GenALEx software revealed a 48% genetic diversity among Juniperus populations and 52% of molecular variance was observed within the populations. The mean percentage of polymorphic loci in each population estimated to be 38.64%. Natural sexual propagation by seeds in each population likely is the main reason for the high rate of polymorphic loci. Another interesting issue is the genetic differences between male and female trees belong to one species which we should take into account during clonal propagation of designated juniperus species. The high rates of genetic variation within population which likely is associated with dioecy and presence of unisexual flowers in Juniperus species and also specific allelic combinations which could have been selected in order to adapt to particular environmental conditions are not present in other areas of distribution are prime importance for planning conservation strategies. By means of assessing the amount and distribution of genetic variability, this study represents an important step towards developing conservation guidelines and strategies for native Iranian Junipers.
Full-Text [PDF 304 kb]   (1292 Downloads)    
Type of Study: Research | Subject: General
Received: 2018/05/9 | Accepted: 2018/06/11 | Published: 2018/11/3

1. Adams, R.P. and F. Hojjati. 2012. Taxonomy of Juniperus in Iran: Insight from DNA sequencing. Phytologia, 94: 219-227.
2. Adams, R.P. 2011. Junipers of the World: the Genus Juniperus, 3rd edition. Trafford Publishing Co. Bloomington, IN, USA. p. 422
3. Adams, R.P., J.A. Morris, R.N. Pandey and A.E. Schwarzbach. 2005. Cryptic speciation between Juniperus deltoides and J. oxycedrus (Cupressaceae) in the Mediterranean. Biochemical Systematics and Ecology, 33: 771-787. [DOI:10.1016/j.bse.2005.01.001]
4. Daneshvar, A., M. Tigabu, A. Karimidoost and P. Christer Ode'n. 2017. Flotation techniques to improve viability of Juniperus polycarpos seed lots. Journal of Forestry Research, 28: 231-239. [DOI:10.1007/s11676-016-0306-2]
5. Daneshvar, A., M. Tigabu, A. Karimidoost. and P. Christer Ode'n. 2016. Stimulation of germination in dormant seeds of Juniperus polycarpos by stratification and hormone treatments. New Forests, 47: 751-761 [DOI:10.1007/s11056-016-9542-8]
6. Douaihy, B., G.G. Vendramin, A. Boratynski, N. Machon and M. Bu Dagher-Khayat. 2011. High genetic diversity with moderate differentiation in Juniperus excelsa from Lebanon and the eastern Mediterranean region. AoB PLANTS, plr003, doi:10.1093/aobpla/plr003. [DOI:10.1093/aobpla/plr003]
7. Hampe, A. and R.J. Petit. 2010. Cryptic forest refugia on the 'Roof of the World'. New Phytologist 185: 5-7. [DOI:10.1111/j.1469-8137.2009.03112.x]
8. Hamrich, J.L., M.J.W. Goat and S.L. Sherman-Broyle. 1992. Factors influencing levels of genetic diversity in woody plant species, New Forest, 6: 95-124. [DOI:10.1007/BF00120641]
9. Hoffmann, D. and F.N. Hoffmann. 2003. Medical herbalism: The science and practice of herbal medicine. Healing Arts Press. 672 pp.
10. Hosseini, S.S. and V. Hosseini. 2014. Effect of Reforestation with Pinus nigra Arnold, Pinus eldarica Medw. and Cupressus arizonica Greene Spices on some Properties of Soil (Case Study: Garan region, Marivan). Ecology of Iranian Forest, 2: 37-44 (In Persian).
11. Jiménez, J.F., O. Werner, P. Sánchez-Gómez, S. Fernández and J. Guerra. 2003. Genetic variation and migration pathways of Juniperus thurifera L. (Cupressaceae) in the western Mediterranean region. Israel Journal of Plant Sciences 51: 11-22. [DOI:10.1560/ABR5-A6MP-5XEG-V0WF]
12. Kasaian, J., J. Behravan, M. Hassany, S.A. Emami, F. Shahriari and M.H. Khayyat. 2011. Molecular characterization and RAPD analysis of Juniperus species from Iran. Genetics and Molecular Research, 10: 1069-1074. [DOI:10.4238/vol10-2gmr1021]
13. Kermani, M., A.H. Maraashi and F. Melati. 2009. Study of genetic variation of Juniperus polycarpus from Tandure National Park of Iran using RAPD markers. Rangelands and Forest Plant Breeding and Genetic Research, 18: 115-124 (In Persian).
14. Kumar, L.S., K. Tamura, I.B. Jakobsen and M. Nei. 2001. "MEGA2: Molecular evolutionary genetics analysis software". Bioinformatics, 17: 1244-1245. [DOI:10.1093/bioinformatics/17.12.1244]
15. Meloni, M., D. Perini, R. Filigheddu and G. Binelli. 2006. Genetic variation in five Mediterranean populations of Juniperus phoenicea as revealed by Inter-Simple Sequene Repeat (ISSR) markers. Annals of Botany, 97: 299-304. [DOI:10.1093/aob/mcj024]
16. Mozaffarian, V. 2009. Tree and shrubs of Iran. Farhang Moaser publication. 991pp (In Persian).
17. Silvia Pinna, M., O. Grillo, E. Mattana, E. Canadas and G. Bacchetta. 2014. Inter and intraspecific morphometric variability in Juniperus L. seeds (Cupressaceae). Systematics and Biodiversity, 12: 211-223. [DOI:10.1080/14772000.2014.904827]
18. Stange, C., D. Prehn and P. Arce-Johnson. 1998. Isolation of Pinus radiate genomic DNA suitable for RAPD analysis. Plant Molecular Biology Reporter, 16: 1-8. [DOI:10.1023/A:1007540901981]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Ecology of Iranian Forest

Designed & Developed by : Yektaweb