Volume 11, Issue 21 (8-2023)                   ifej 2023, 11(21): 159-169 | Back to browse issues page


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Rousta M J, Matinizadeh M, Nouri E, Zarafshar M, Enayati M. (2023). Investigating the Diversity, Abundance and Degree of Symbiosis of Arbuscular Mycorrhizal Fungi with Trees and Pasture Plants in Kowsar Station. ifej. 11(21), 159-169. doi:10.61186/ifej.11.21.159
URL: http://ifej.sanru.ac.ir/article-1-491-en.html
Agricultural Research, Education and Extension Organization
Abstract:   (1567 Views)
Extended Abstract
Introduction and Objective: Arbuscular mycorrhizal fungi, due to their very beneficial effects in the growth and development of plants through supplying the water needed by plants, especially in drought stress conditions and increasing their tolerance to salt stress and in general, helping the plant to overcome living and non-living tensions, they are the focus of many researchers. This research was conducted during 2020-2022 with the aim of investigating the effect of flood spreading, vegetation type on the abundance and diversity of mycorrhizal fungi in Kowsar Station.
Material and Methods: In spring and autumn, in different land covers, including planted Eucalyptus and Acacia forest, planted pasture with Atriplex bushes with 37 years old and natural pasture in two situations with flood spate irrigation and without spate irrigation. Three samples were prepared from roots less than 1 mm of trees and dominant plants (Heliantemum, Dendrostellera, and Artemisia) in the pasture and the rhizosphere soil up to a depth of 20 cm. After separating and staining the roots, their microscopic examination to determine the presence of symbiosis in the root tissue and to determine the extent of root colonization with mycorrhizal fungi and the extent of mycorrhizal symbiosis based on the extent of root contamination with structures of mycorrhiza was done. The soil passed through a 2 mm sieve was used to separate the spores, and the genus and species of mycorrhizal fungi were identified based on the morphology of the spores using identification keys and information available in scientific sources.
Results: The results showed that the spore density of mycorrhizal fungi in both sampling seasons was higher in areas with flood spreading than areas without flood spreading. The highest spore density in the field of flood spreading in the autumn season is related to H. lippii with the number of spores 50.20 per gram of soil and the lowest with the number of spores 18.80 per gram of soil related to this species in the field without flood spreading in spring. So that the difference between the two was statistically significant with Duncan's test at the 5% level. Also, in all land uses, spore density was higher in autumn than in spring. The highest percentage of root colonization or the percentage of symbiosis was related to Acacia with 61.81% in the field without flood spreading in spring season and the lowest value was related to Eucalyptus with 12.26% in the field without flood spreading in autumn season. In this research, 21 species belonging to 9 genera Acaulospora, Claroideoglomus, Diversispora, Entrophospora, Funneliformis, Glomus, Rhizophagus, Scutellospora and Septoglomus were identified in with and without flood spreading areas. Septoglomus constrictum was the most abundant with 100.0% frequency. Glomus heterosporum and Rhizophagus aggregatum were in the second category with a frequency of 50.0% and Funneliformis mosseae, Glomus ambisporum, Glomus intraradices and Rhizophagus fasciculatus were in the third category with a frequency of 33.3%.
Conclusion: Considering the fact that the identification of mycorrhizal fungi in the rhizosphere of tree plants and pasture bushes in dry areas and their use can be a fundamental step to reduce the negative impact of various stresses, including drought stress, it is suggested the active and effective species of these fungi may be identified and propagated and used as inoculum (biofertilizer) to restore rangelands in these areas.
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Type of Study: Research | Subject: Special
Received: 2022/12/19 | Accepted: 2023/01/7 | Published: 2023/08/1

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